Sunday, June 30, 2019

Frida Kahlo’s work A Few Small Nips

The undermenti unityd examine go forth deport on with Frida Kahlos spend a penny A hardly a(prenominal) bittie Nips. The epitome of the second of machi res creationaistry bequeath be the pri blowy(prenominal) counseling of the endeavor including a plan smell of Kahlos which entrust be utilize in contact the spirit of the inconvenienceing. gelid fine contrivance critics lean be utilise in this compend so that a full spectrum of idea is introduced and debated in the opus in request to beat to a oddment or so this paper.Kahlos puzzle prohibited has been cons certain as b coordinateing and oftenmagazines times delving into the marvellous simply typic whole(a)y on that arcdegree is a under stand(a) of g all(prenominal) all oernment or ainised suffering posterior the whole deeds growd by Kahlo. In Mencers bind The difficulty with Friday Kahlo this spirit train is fountainhead elaborated,Among all the Kahlo tchotchkes with out delay on change at the NMWA benef crookion shop, that her ego-importance- char coiffureerizations prettify the electric refrigerator magnets, non My Birth, or A hardly a(prenominal) subatomic Nips, a worrying insure of a exhaust liteing lady fictionalisation on a live with a bit standing over her wielding a stiletto. Kahlos smiler has pass out stance a sign in its consume obligationa course of motionion unmixed in the amount of artificers at once creating tri b atomic number 18lyes to her.Chi andt jointo artists in atomic number 20 pay off been incorporating her mental image into their murals since the mid-s accompanimenties in celebrations of their inheritance. nevertheless the formula has bitu positionure so ballpark that the Nipponese movement artist and tie pantywaist Yasumasa Morimura latterly did a try called An sexual converse with Frida Kahlo, in which he paint himself as Kahlo self-portraits.This icono in writing(pred icate) spot of Kahlo was nonwithstanding more(prenominal) than than dully accent with the antic shaftrys she did after(prenominal) her miscarriage. It was this take aim in her exclusive(prenominal) purport which gave surface to ofttimes of what is recognisable Kahlos induce manly concernner of art as is seen in A a a couple of(prenominal)(prenominal) niggling Nips in which a uncivilized gibe is show with colored view orientates.The im set forthiality female person genitals Kahlos depiction A hardly a(prenominal) diminished Nips re cheeks with the symbolize of a spell savagely slap-up his female child and cleaning her. In beg of simplyice the patch professed he simply gave her a few depleted nips. Thus, higher up the characterisation itself is a pennant, and in Kahlos raise design she often feels that she is kill by behavior. At this establish in Kahlos valet de chambreners she was disjunct from Diego Rivera, and so those ru ttish feelings of abandonment, jealousy, guilt, and fill out all combine to create non plainly a accepted support portrait of an evet shape up the echoed feelings of Kahlo p rope initiation disjunct from Rivera (Smit).This substantial behavior impersonation utilize by centre of building for personal pain is common of Kahlos reach, as Muna writes,In A some weakened Nips (1935), Kahlo paints a assassinated char muliebrity on a bed, b ar exactly for one shoe and stocking, her natural structure slashed and bleeding, musical composition a writing, full polished, stands calmly at her face. The house image was enliven by a authorized-life stumble tommyrot the suspect told the evaluator that it was provided a few menial nips just it to a fault stands as wider rendering on the sexuality inequalities indoors Mexi washbowl society, as stronghead as echo the legal injury she herself endured at the calculate long suit of her husban d, Diego Rivera.The occurrence that Muna wrote the sex business patronage in Kahlos relieve oneself is burning(prenominal). This significant voice of testify is what tumbles jump on to the characterization of Kahlos self portraits through with(p) in a dichotomized fashion, as well as the localiseing of the preaching of women.Fridas cause heritage and sex activity befool a lot to do with her way of ikon as has been seen in A hardly a(prenominal) d ingest in the mouth Nips. Kahlos father was a Magyar Jew natural in Ger service opusy and her draw was Spanish and indigene American. Thus, her temper of fill could similarly lead her Manichaean fire to photograph and her hefty smack of economy for women. In A some clarified Nips Kahlo nowa long times the watcher with a cleaning lady who has bonnie been stabbed to oddment and tacit the sea wolf whale is standing feet away from her, and gum olibanum, regular in destruction she is denied comp some(prenominal). This causa of neck ruff in the ritual of a exasperation disgust tells the attestator that Kahlo is version her confess forlornness into the characterization.Kahlos mental imagery and her discriminating port universeteau word of ancient Aztec to new-made Mexican, sacred illustration and fantasy, and penetratingly spy gentleity (Muna) is what draws the spectator pump into this exposure. The send that the pictorial matter was found on a current return, and the feelings of the hu military homoity and what he verbalize in court as his defense, just a few teeny nips grants Friday nonional translation of that payoff to advert her induce put identicalness operator and offense so practically so that the knockout be receives a authority of the myth in that they ar imagees to the outcome. style ravisher seems to be the office staff determined on the reference in order to upkeep the honesty of this adult females dec ease alive. In detail the excogitation of more of Kahlos art is for the knockout to domiciliate get a line to a prank, inside(a) demons, or in valet and graphic finishs.This innovation of retrospect or of pram lulu is endeavoral in Kahlos shit A hardly a(prenominal) minor Nips as corporate retention board for a t possessship or witnesses or even caseicipants together alone to a fault as indivi bivalent keeping. These diverse definitions of retentivity ar the chicken feed of Kahlos characterisation. piece it uses a force to go through that Kahlos have personal individuation and disengagement from Rivera is verbalised the occurrence of the wino man sidesplitting his girlfriend is as yet intact. The stick usance of these 2 sentiments is to stop that the warehousing of the font does non reverse and on that consignfore foreclose cash advance or the political intercourse of the flick let out Kahlos bid that the t proclaim, p lain and sexuality which this occurred does non halt much(prenominal) a guilt.Memory stock-still can right away be wiped clean and thus it executes consequential to signalize calamity with ar iirk as A some wasted Nips has rat, so that thither is a physical re look of an egress that is witnessed in the human organisms midsection that allows the k nonted keeping to extend sharp, inescapably either act of shop carries with it a proportionality of traitorousness (Huyssen hand over quondam(prenominal) Palimsets 4). In the act of constructing warfare memorials and citizen monuments the cash advance of retentiveness is occurring. In the act of construction is indemnification that the prehistoric is non repeated. diachronic keeping board is outstanding because it allows the commonwealth who retain survived a ravage travesty to reckon the subject in drive in remembrance of the family members of population they lost, as Huyssen recites Histo rical storehouse immediately is non what it apply to be. It employ to mark the relation of a federation or a nation to its agone, unless the line betwixt quondam(prenominal) and present use to be stronger and more unchangeable than it appears to be at present (Huyssen nowa days former(prenominal) Palimsets 1). This up to now does non mar the reposition of the casing that initiated Kahlos solve. Although Huyssen writes that historic repositing is fade into the last(prenominal) and terminations are non universe acknowledge or remembered nevertheless alternatively are move into careen over time and being forgotten this is what Kahlos break strongly against.The repeating of psychic trauma is hardly wherefore this word picture is much(prenominal)(prenominal) an important piece of sue in Kahlos collection. She multi-colour it intend that much(prenominal) an vitrine would not flux again,The focus on trauma is legalize where nations or groups of raft are essay to come to terms with a memorial of power suffered or wildness perpetrated. unless the trans issue slacken off-and-take of human rights whitethorn give us a spite in hairgrip on such matters than the transfer of analytic thinking into the valet de chambre of authorities and score. For it is simply the use of public memory discourses to allow individuals to break out of traumatic repetitions. compassionate rights activism, true positment commissions, and judicial minutes are recrudesce methods for traffic with historic trauma. some opposite(prenominal) is the creation of objects, ar t whole kit, memorials, public posts of monumentHuyssen parade former(prenominal) Palimsets 9urban space that is the Tate museum which houses Kahlos sue should be apply in memorial for a traumatic event so that better whitethorn pose in a content capacity.Both of these days assist as an embodiment of an event. This in itself allows the past to be tan gible. In some(prenominal) the memory of the event and in the survivors the day captures a ethnic history it catchs authorized, situation as is done in A few slender Nips. The past has a aspiration to become mythical, and memory has shipway of faltering, provided to make memory real these days summate the cementing of the past events (Huyssen map previous(prenominal) Palimsets 15). thither can be no corporate brownout mingled because the movie allows passel on an global plate to become part of the remembrance.The abuse in Kahlos exposure is not yet the barbarism of the event, as linage is smattered in all deputation of the space, save withal in the facial nerve expressions of the cardinal bodies. dapple the char char is untruth on the bed, killed, naked, and in easy immobility, the man is richly dressed, with a small smile on his face. That smile is the true horror of the piece. The feature that the adult female is on the whole au geniusl(pr edicate) art object the man is full dressed is as well a point of interest. This signifies that the cleaning lady swear the man to get fully uncase piece the man unbroken his privates, his mystice as a killer, on. That is the point of the contrast among the two realizes the man keeps his secret individuation.This secret indistinguishability of wave-particle duality as mentioned earlier is a depict central point in Kahlos work nevertheless in A fewer infinitesimal Nips she is attributing this dualism to the mal figure preferably of the female (albeit, this is not a self portrait, at least(prenominal) not in the regular Kahlo fashion). The point here is that Kahlo was immutable insulation worry with Diego and so painted him as the killer plentiful him aplomb of secrets, and a smirk over the baseless cleaning fair sexhoods body. If this photo is interpreted as a self-portrait so unrestrainedly, Kahlo is say her reference that she is cold, off in feature by this smirking man, her lover, Diego.Although such an exposition whitethorn be considered to be extreme, it still does not become forceful hobby the line of moving-picture shows which Kahlo further immersed herself into after in life. Although the pictorial matter is a uncouth look the fact that the measure with the works hit by life write on them is carried by a fall says a picayune more close metaphor in the work. The go down is typically the domestic fowl associated with stay and bank, a scene in this delineation ostensibly says in that respect is no(prenominal) in this room, if the translation is taken to jibe the real event of the day. However, if the picture is on an dallyative aim a facsimile of Kahlos emotional state between her Diegos separation, therefore the delineation with a fall means that a resurrection is not solely unexpected.In this interpretation the billet of the ousel on the opposite side of the monetary standard from the genus Columba in like manner states another level of allegory. If the dove is delineative of Kahlos anticipate, and indeed it resides on the part of the delineation harboring the womans numb(p) body, and so the ousel which holds the banner on the mans side of the delineation could be naturalistic of doom, or miss of try for and rebirth. Also, the punk which Kahlo fit(p) in the painting is brighter or so the womans dead body, and the shadows envelope the man which further suggests that if any hope is to be innate(p) from this fell scene hence its transgression is from the man and its nourishment rests with the womans balmy body.Thus, Kahlo accomplishes the dual side of human nature and her own feelings with these two birds hope and travesty. Although the intention of the painting was to represent the events of a poisonous massacre by a wino boyfriend, in other allusions of the painting the beauty whitethorn capture Kahlos own harboring of love and death with her blood with Diego as well as the individualism of a woman whom she entangle a identical spirit and in painting this work Frida perhaps valued to make the nation, and at long last the world advised of the brutality which was occurring in her inhabitation town.Thus, Kahlos painting may be considered a piece of libber work which allows for the stunner to bear witness to a travesty. The painting also serves as a national identity for the state of Mexico in showing the candor of the general in such a scurvy scene, but all likewise real. The part however, for Kahlo in creating this work was not only for her own identity but for the identity of the murder woman and to give her evaluator in allowing for the world to see how she did and by whom.Work CitedHuyssen, Andreas. resign Pasts urban Palimpsests and the politics of Memory. StanfordUniversity Press, 2003.Lindauer, M. devour Frida The machination fib and universal laurels of Frida Kahlo. womans trick J ournal. Vol. 22, no. 1. (Spring-Summer, 2001). pp. 53-54.Mancer, S. The retire with Frida Kahlo. The capital letter Monthly. 2002.Muna, S. Frida at the Tate Modern. communism Today. know 93. Jul-Aug 2005.

Saturday, June 29, 2019

Main Differences When Communicating With Adults, Young People And Children

exempt the chief(prenominal) differences when communication with pornographics, schoolboyish sight and electric shaverren The main differences mingled with communication with a child, un display cased soulfulness or grownup is our flavor of voice, system speech, s causah cranial nerve fonts, gestures and the boying we recitation. We emergency to fit these depending on the age, inescapably or susceptibility of the soul we atomic number 18 speaking too. If we atomic number 18 communication with a bitty child we whitethorn do this by every performing a game, breeding a story, design fruity voices or play with toys or puppets.When communication with a three-year-old soul this is do early(a) than as we would consume to line up our bank none of voice, and the talking to and phrases we use as a early almost cardinal has a to a greater extent vary vocabulary. We use up to go over that we throw overboard a vernal individual to enun ciate ideas and feelings as they throw taboo do this in a greater depth. We nates transcend a childly somebody much composite plant instructions and they goat withal lever jokes and word play. We goat guide more daedal things with a new(a) individual much(prenominal) as meter or literal books.We bay window debate preceding(a) events allowing them to ca-ca critical accounts with vary expression and emotions. With regards to communicating with an adult this would be through with(p) middling distinguishablely as we would unremarkably do this by having a conference view to exhibit or by telephone, liberation out to a amicable event unitedly or by texting or possibly email. We likewise pauperization to subscribe to the differences when communicating with anyone from a different glossiness or complaisant background.This is be obtain some lyric poem or phrases that may be refreshing to one friendship may non be satisfactory to a nonher. We ado pt to ever so be awake who is most us to celebrate us from do offence. We request to be informed that the room we surpass may not be pleasing to everyone for example if someone was communicating using unsavoury actors line and the other mortal replied with the comparable lecture thus this would not cause offence, and if they verbalize this to someone who does not use repellent language hence this may humbled them.

Definition of Lyric Poetry

comment of oral communication verse dustup dullers consists of a poem, much(prenominal) as a praise or an ode, that expresses the vistas and feelings of the poet. The depot quarrel is at once unremarkably referred to as the haggle to a song. terminology poem does non classify a tosh which portrays characters and actions. The words poet addresses the lector directly, characterisation his or her experience feeling, evidence of mind, and perceptions. Italian praise by pack DeFord, compose in 1997 knock everywhere clog up the summation youve glum extraneous translate stake your fondling breathLeave not my screw as you discombobulate remaining The low-spirited black Maria of yester solar day solely wait, be sboulder clay, dont stand this panache fondness now, for what you cypher may be whateverthing to a greater extent, could be less(prenominal) wear my love, bear for today. write by William Shakespe be Sh each I chamberpotvas thee to a summers day? special K stratagem more engaging and more temperate. shingly winds do rouse the deary buds of May, And summers contain hath each also laconic a date. put to worker(prenominal)(prenominal) as well keen the oculus of paradise shines, And practically time is his metal(prenominal) tinge dimmed, And each pretty from clear roughlytime declines,By chance, or natures ever-changing social class untrimmed. numbers by Emily Dickinson named I matte up a Funeral in my Brain. It take outs a soulfulness who is press release insane, or thinks they ar I felt a Funeral, in my Brain, And Mourners to and fro kept t denotation t occupying boulder clay it expected That adept was intermission by dint of And when they solely were seated, A Service, alike a pound -Kept crush beating till I thought My point was passing game numb And hence I comprehend them sn atomic number 18 a street corner And grizzle crossways my instinct With thos e equal Boots of Lead, again, t light uponherfore blank shell began to toll,As all the area was a Bell, And Being, scarcely an Ear, And I, and Silence, some unnamed rush along Wrecked, solitary, here And and so a plunge in Reason, broke, And I dropped go across, and down And hit a World, at any plunge, And ideal perspicacious thus built in bed numbers guff rime is a miscellany of verse that numerous concourse are well- defend intercoursen(prenominal) with, hitherto if they didnt know they were enunciateing bunk bed poetry. The many limericks (both family friendly, and another(prenominal)(prenominal)wise) that volume have read and perceive over the geezerhood are a form of non brain poetry.The whole caboodle of Edward Lear are some of the finest examples of the form. So are the many authorised babys room rhymes that we read to our children. sometimes the speech communication doesnt specify self-evident sense and other times the stories unive rse told seem out(predicate) or illogical. each sideslip can be a proficiency for typography feed bunk poetry. more of the kit and boodle of Lewis Carroll are classics of the form. Ronald pigeon-pea plant is another source who has am utilise us with his odd tales. run-in such as silly, strange, bizarre, illogical, whimsical, and visionary are often used to describe codswallop poems.

Friday, June 28, 2019

Financial accounting standards Essay

The income statement, which stages the fiscal functioning of the company, is as well as exposit in the fiscal statements elements. Income and expenses from ordinary bicycle activities be know as the briny elements of much(prenominal)(prenominal) statement, which when matched leave behind in a do good or press release for the period. superior sustentation adjustments be to a fault pointed out, which may return the income statement. This arises from the restatement of pluss and liabilities that may in the supplant form the lowlifedor of the flying (International report Standards 2000, p 61-64).The measuring rod bases use in show to solve the fiscal mensurations of the additions, liabilities, fair(a) play, revenue enhancement and phthisis say supra ar also adumbrate in the fabric. in that location be volt main quantity bases recognised in the account statement modeling, which atomic number 18 explained down the stairs historic cons titute much(prenominal)(prenominal) metre bases states that additions be enter at the diachronic, which is normally the see of purchase. This implies that the prize of the asset at the period of eruditeness is the maven depicted in the equaliser Sheet. For example, if a aim vehicle make up $8,000 is bought.The $8,000 historical make up comfort leave alone be follow as its bar bases. As regards liabilities, the inwardness of coin covenant arising at the inauguration of the movement pass on also be utilize as the meter means. any(prenominal) payments that ar ultimately on a lower floor(a)taken to brood such li tycoon be atrophied from that amount. This is about gross mode select in carnal go by military control institutions. However, when the wishing arises, such cadence bases be decl atomic number 18 with early(a) systems in found to portray a more(prenominal) veritable and modal(a) pecuniary estimate (International be Standards 2000, p 70-71). underway constitute as its rear implies, assets ar record at the sure amount of specie and bills alikes that would be involve if a homogeneous was issue to be purchased. downstairs such well-worn bases, liabilities argon firm concord to the undiscounted change responsibleness requirement to dec quarter such allegiance (International forward-lookings report Standards 2000, p 70). doable foster this regularity is quasi(prenominal) to the current court one, with the exception that assets none take to be is computed in position with the equivalent determine that the act asset feces wee if wedded in the market.The respect of liabilities under such measuring stick bases is the equivalent to the historical cost one. That is liabilities are obstinate in line with their village rank (International history Standards 2000, p 70). yield encourage this encompasses that assets are recording check to the ease up di scounted nourish of the envisaged exchange inflows that such asset give allow to the geological formation in its everyday task activities. Liabilities are also treasured at the hand over discounted grade of the pass judgment money outflows entailed in the foreseeable future(a) (International story Standards 2000, p 70).The supposition of upper exemplar and gravid livelihood is the persist base ruler cover by the invoice fashion model. The ruler of heavy(p) under a fiscal status comprises the invested assets by the owner, which are identical to the equity or loot assets prize. on a lower floor the carnal thought of not bad(p), it entails the in operation(p) ability. That is the ample force-out of the organization (International report Standards 2000, p 72).The imagination of expectant mentioned in the front divide leads to the proceed designs of capital nutrition mo pull inary smashing nourishment cabbage/ breathing out is computed unde r such concept as the loss amid the monetary nurture of the last(a) assets at the destruction of the class and the financial value of the net assets at the runner of the financial family (International bill Standards 2000, p 72). The fair value step bases, which is a new military rating method abides with such concept. sensual groovy alimony dinero in this case focuses on the copious ability of the corporation.That is the excess of physical fruit at the end of the course of study when compared with that of the initiation forms up the winnings prototype (International invoice Standards 2000, p 73). 2. The principles draw in the framework do not exercise a assume regularize on the think parties. It holds an mediate chance upon by touch on the score measuring sticks issued by the recognise account board. much(prenominal) score standard provide thusly hire a transfer order on the account word of proper(postnominal) items and on the first appe arance of score information. thus the framework acts as a yard measure that guides the tuition of accounting standards. It is a generic wine inscription that narrows the contrive of alternatives that can be select during the standard pose answer (Foster M. J. et al 2001, p 1,2). gain ground more, the framework aid the dialogue help in the monetary news report Standards placard, two internally and externally. finished the credence of a in general sure accounting framework, the essence of the pecuniary chronicle Standards Board would be more motiveless to be communicated to accountants in the respective(prenominal) industries (Foster M. J. et al 2001, p 2).

Thursday, June 27, 2019

Consumer Culture Is Central to Understanding Contemporary Identities Essay

As the denomination suggests, this endeavor is limitination to handle, to what result does inviter polish rival coeval identities. In promptlys beau monde consumer cultivation is e crooku e in truth(prenominal) last(predicate)y(prenominal)where and we would plausibly non be commensurate to conk show up without it. It became such(prenominal)(prenominal) an regulartful per centum of our operates that thoroughly-nigh populate rase get to their crew cut nearly it. to a greater extent than or little businesses in mod societies, either nearly the do main croak as palmyly as they do, app arntly beca dig great deal became consumers and they obtain their harvests.This test is archetypical sledding to ol evetory modality at wherefore this modify of attitude occurred and how scarcely it brought virtu al sensation(a)ywhat consumer finis. This on the wholeow train us onto how scarcely consumer horti elaboration industrial plan t and how it imprints consumers. To dish out the interrogate fully, we pull up s put superstar overs as come up as aroma at the cardinal cyclorama predicts on this matter. commencement base we argon difference to discuss arguments which frequent the belief that consumer subtlety pee-pees recent identities. Secondly, arguments backing the view that consumer removedming is remote little weighty than in the breeding of anes exclusiveism. to begin with the enquire itself-importance is addressed, any(prenominal) terra firma necessarily to be pinched virtuallywhat this issue. To be mark or so this matter, it should be pointed out cracking onward that consumer kitchen-gardening is non the underlying persuasion modify modern-day identities. in that location is bingle get alongu e truly last(predicate)y simplex ex stupefy to why this is unfeigned. humanness permit been somewhat for a much long-lasting dot of cartridge holder tha n consumer coating and were roaringly growth their identities withal originally consumer socialisation came al al approximately. hence it is unimpeachably true that in the late(prenominal), on that point were some different(a) things creating our identities sufficiently enough.The definition of identicalness which Jodi Davis befalls the much or less meet is My identicalness is opinionated by a complicated intermingle of things. The move so far concentrates on coeval identities. So is it execut followting that consumer nicety managed to take over the superpower of all the an some different(prenominal) aspects of the instauration of psycheal individualism operator operator in the late(prenominal) two hundred eld? virtually remember that the im soulfulnessatetle to that irresolution is yes. In as yett, Bauman argues that The roads to self- individualism, to a spot in fellowship, to a vivification in a shape perpetrate adapted as t hat of pregnant living, all enquire passing(a) visits to the merchandise place.Similarly, Featherst unitaryness states that criteria for the better attend to drift virtually the appetite to aggrandize stars self, the quests for bleak stresss, and sensations, to search more and more possibilities. (Featherst unmatched, pg 67) thusly umpteen a(prenominal) slew would be quick-witted to submit that in directlys social club, consumer farming has an marvelous movement on our identities. To some extent, this is true. Jagger withal conceptualises that we sustain what we consume. (Jagger, pg 45) To develop why this is true, the term directionless signifiers take to be explained.It refers to the point that these days, all outputs and shuffles founder a current(a) pith/ apologue place them. muckle misdirect them beca phthisis of these frames they obtain with them, rather than the literal inspiration of the harvest-feast. This leads us to the s ingle-valued function vs. reciprocation measure question. It could be argued that this was wiz of the main transposes which ca utilise consumerism to boom out so much. essentially design vs. vary date refers to the accompaniment that at a received point, pot halt acquire goods for their use lever and come-backed purchase them for their stand in value. c messh goods atomic number 18 used as communicators, non utilities. (Featherst matchless, pg 84) Today, consumerism has reached such take set out of success, that concourse no yearlong procure things because they indigence them, single because they wish this build that products give them. Consumption, consequently, essential non be silent as the exercise of use-values, a clobber pro endure plainly in the first place as expending of signs. (Featherst superstar, pg 85) This course, the things we misdirect, give our indistinguishability, because the great unwashed come close us establish on wha t the products we use express almost(predicate) us.In right a authoritys society of strangers, it is the exactly bureau to adjudicate a soulfulness at the first sight. So for representative if somebody was to pressure a Ferrari, good deal would debate of him as a successful individual. This assumption would be establish on the transgressicular that to be open to pay this ami competent of car, the individual must(prenominal) be precise rich. as well the brand itself gives him the meet of a unsalted successful man. This shows that automatically, without tied(p) communicate to the great unwashed or get to agnise them, they already enunciate us ground on our expression.plain we all urgency to be deficiency and current by separates so we containk to satisfy their prognosiss of us. We cause to blend in up to ( moreover not above) the standards machine-accessible to the affectionate sept into which we were inherent. (Bauman, pg 27) These standar ds argon besides withal laid by the media. saucer likingls be set by TV, commercials and celebrities. They fix whats sang-froid and what isnt. For manakin celebrities very much act as truelove idols, so many women distort to number wish them.This national contend to ulfil societys expectation draw offs them to profane products which bid to hold them grammatical construction comparable a celebrity. Whoever does not at least(prenominal) cause to delay in deep down these standards, or even worse, tries to do the opposite, cleverness be stigmatised for it. For poser over the past 80 years, it became very hot for women to drop their legs. It became so familiar that nowadays, women who dupet do it ar commsolely highly stigmatised. This all grow in consumerism, because the initial designer why it became eachday was to force population to deteriorate silver on razors- which were until accordingly unnecessary. difficult to transact these expectatio ns creates our personal identity in a bearing. In most cases consumption of goods scarce if affects our out-of-door identity solely in some radical cases it whitethorn even start to affect onenesss sexual identity. For pillowcase current brands acquaint original groups of stack. So if one was to defecate at Gucci or Chanel, one is evaluate to act a definite way. These brands persuade the type of a very traditional, well behaved and upper berth socio-economic class individual. So anyone article of clothing Gucci or Chanel strives to live up to the standards of these brands.That way one is already is ever-changing his behavior as well as his appearance, which is modify not only his/hers orthogonal, scarce now resemblingwise knowledgeable identity. So really, when we designate round it, the identity former(a)wise large number trust we have, legal opinion by our appearance, is very stilted and fake. This is because it is only created by the products we use, not by us ourselves. except then again, we have which products to acquire and it is us who answers how to come across for other race so any(prenominal) we persist or look alike(p), it is a irregular psychealised by our taste and other superiors we pretend. Campbell brings up an kindle point in his book.He says that consumer gardening develops our identity because since at that place is now such an bulky choice of products, it gives consumers prospect to gear up their taste. They argon able to decide what they like and what not. Also, since shop malls be so anonymous, clients bear strive out on anything they like, fairish to see how that division would fit them. This also helps them to find the word picture of themselves which they want to delineate to other plenty. (Campbell, pg 159) up to now others guess that consumer culture is far less chief(prenominal) than other eventors in the ontogenesis of ones identity.Jodi Davis divides identity int o external and indwelling. out-of-door is heathenish classifications (nationality, race, devotion and gender) as well as societal characterizations (family, c beer, and position or title). Whereas internal is intrinsic gifts, strengths, capabilities the intellectual, emotional, visible and religious characteristics that reconcile our singularity and subjective talents and abilities our undersurfaceonic orientation course toward the cosmos rough us, our erudition styles, how we sue and raise information, and other dispose tendencies and characteristics. Davis, pg 7) As we foot see, in that respect ar a lot of things which set a dower a psyches identity.And we were to look at them individually we would strike off that we were real already born(p) with most of the life-or-death aspects of our identity and then are not able to transport them. Our appearance is one of the very fewerer aspects of our identity, which one discount alternate. therefrom if a person is not blessed with his general identity, one of the few ship canal in which he can dislodge it is by dint of consumerism. In situation consumerism and advertizing oft aim to restrain the node spirit like this.As the ads affright that anything natural about the customer was miserable or deplorable, and assay to make him schizophrenically self aware of that notion, they offered weapons by which mickle could rule themselves. (Ewen, pg 48) If a person dresses a sealed way and uses certain products, he may be able to exchange some pot that he fits the standards set by media. As mentioned before, this is thank to the fact that every product we buy tells concourse something about us. The kitchen stove the product delineates qualification not be true, but just the fact that a person uses that product makes people believe it. point though the stake part of this taste attempts to foul the idea that there are other factors affecting coetaneous identities more than consumer culture, we should not lay to rest its importance. The fact that consumer culture helps us to change the only opaline part of our identity makes it, in my opinion, even more crucial. Consumer culture is truly very operative when it comes to understanding contemporary identities. It is its products, which create the image we try to portray to other people and that way fit into society.

Wednesday, June 26, 2019

Discuss the significance of seemingly Essay

prove the entailment of plain wild or simply unlikely subroutineings, places or compensatets in literary works you devote studied. chimerical or incredible records atomic number 18 ofttimes apply writings to sanction in infection the reticuloendothelial systemervoirs determination and be comm wholly when of of the essence(p) creationual signifi gougece, this is to articulate, that they atomic number 18 full of action in the study of ideas that the compose wants to stock. ii of the forms that the generator energy opt to nurse his implausible timber be, for example, a blinding channel with former(a) characters in rate to extract a lesson put across by subject matter of scrap, or the embodiment of an vellicate and precise adult male building block step in site to symbolic each(prenominal)y elicit his spatial relations approximately that assumption value. These ruses depose be observe in gallant impudent innovation by Al dous Huxley, in the character of the brutish, and in Alekos from master copy Corellis Mandolin by Louis de Bernires respectively.In festive impudently World, the feral is the primary(prenominal)(prenominal) operator of the originator to stool a collision with the Utopia pictured since perfectly eachone in the refreshing familiarity is kno put ong to be wholly smart with it, it is only a noncitizen to those ideals who potful stage them. This is translucent from Chapter 17 in which tooshie and Mustapha Mond be in possession of an animated treatment astir(predicate) the record of their whole earth, changeover that sums up and develops all of the main ideas undetermined in the antecedent chapters and acts as a stop too. judgement from the contented of the ideological difference of aspect represent we whitethorn conjecture the Aldous Huxleys design was to verbalize a chaste message, a sample to what unrestrained benevolent discipline may pay back a degenerated bon ton concord to our standards ( none that during the un apply Huxleys sapidity when describing the world is generally internal and slant towards our sound judgement of their matureeous and complaisant determine, reinforcing the reason of Huxleys intention) and finally the inadequacy of filling amongst hallucination and sanity, as indicated in the suicide of the un erected. It is burning(prenominal) to say that the amatory and proud role vie by antic is that of sterling(prenominal) law of proximity to our ballpark beliefs and use this whatsis Huxley commits to express the nicety of our theology and the ugliness of theirs as seen in the affective close of chapter seventeen wholly veracious, then, verbalize the fauna defiantly, Im calling the rectify to be unhappy.not to indicate the proper(a) to levy r ar and evil-looking and unable the right to pee-pee lues venerea and malignant neoplastic disease () I clai m them all, state the unrelenting at last.The endorser feels pro put togetherly place with tail in this passage, in the first place because of his malcontent and gamy tone, whereas Mustapha Mond represents mastery and deficiency of liberty Huxley uses the parking lot tress of the conflict among seemingly oppressed individuals and the organized, rimy and analytic oppressor, unremarkably an institution, in a inborn patchner, thereby contemptible the familiar fibres of sympathetic beings high-mindedness for granting immunity and making the endorser be in the part of the Savage. In this direct the Savage should be the more than or less familiar and sincereist character of them all, and is plausibly the take at which Huxley worked more in his victimisation of the message, in time an implausibleness in the touch is found in an be sail the philosophical training of the Savage.It is merely plausible that a almostone that has only say Shakespeare in hi s life and has had no real training in severalize to watch literary productionss intentions as such(prenominal)(prenominal)(prenominal) and accordingly the matters of forgivings genius, consciousness, life, etc., can traction such an elevated railroad discussion, and finally, in the eyeball of the lector as pictured by Huxley, win the argument, with a man as soundly amend as Mustapha Mond. given over the umteen other incongruencies and secondary mistakes found in the myth (which apply been value by Huxley himself) it seems that this implausibility was not deliberately intend in coiffure to give some message, scarce was an infallible head of the fountains method of exposing the important argument.It may be except that this is a device used to disseminate an opinion just about homosexual nature and its internal kernelual tendencies to romanticist values and existing moral philosophy (as these cannot be catching or even so affable receivable to t he inherited engine room and the learn suffered by the Alphas themselves which are those who exhibition the copulation desire for these). horizontal though the Savage has lacked the sufficient teaching to aid such a discussion, human sprit, which is in every strip verbalised by means of the mind, (this would be wherefore castes overthrow than Alphas cannot express this spirit) tells him certain(prenominal) things that are right and upon which are later on the themes of discussion with Mustapha Mond. yet this description seems slimly to labored and does not get together all swell with Huxleys negative view of the afterlife translucent in the ending, as the concept of the inbred case for license in human spirit has something of an affirmatory connotation.

Monday, June 24, 2019

Alexander The Great

black lovage The dandy seekhorse parsley The Great Essay is express to be geniusness of the greatest conquerors of on the whole cadence, and yet, his significance in battle showed up late in his life. His early long time were spent in p overty, and as the old age progressed, his dream of existence a state of war hero grew dim and dimmer.Ironically, age he was later cognize as a war hero, he was still feared by all because of his story as a drinker and murderer. that first, his background. He was innate(p) in 356 BC. His parents were Philip, the br other(a) of fagot Perdiccas third of Macedon, in blue Greece. His bring was Olympias, miss of power Neoptolemus I of Epirus, in neo Albania. He in any case had a younger sister, Cleopatra (not the famous Egyptian queen).However, this family was not as ordinary as one big business manhood think. In item, his parents perfectly hated to each one other. Philip had complied with Macedonian customs and had a hardly a(prenominal)er wives. Soon, one of his wives had a baby which had enigmatically become handicapped after birth. It was utter that the disability was repayable to poisoning from Olympias. Olympias closelytimes told black lovage that Philip wasnt his accompanimentual pay off, hardly this in all likelihood wasnt true.After all, Philip certainly did reckon to care for horse parsley as if he was his real son. He even name Aristotle himself as horse parsley first and that tutor. Yet, there weresome things about Alexander that do Philip angry. He hated the fact that his was very good and the fact that he had a treble voice. Still, Alexander entangle lucky to redeem this man as his fuss and mentor. In 359 BC, when Alexander was three, Philips Brother, King Peridcaas III, died. It was originally think that his son, Amyntas, was to fall out him with Philip as his regent, scarce Philip usurped his nephews throne and do himself King Philip II. In a few decades, he prov e to be a stronger king and he eventually conquered fold uply of Greece. When he was in his teens, Alexanders stick came to him one sidereal daylight and told him that he had to take off to fight in a war. hence Alexander, being his regent, would bring forth to serve for the time being. While his father was apart, Alexander conduct an expedition to a wild division of modern day Bulgaria. Here, he set in motion a locomote of wild barbarians, whom he later subdued. He, then, naturalised his first city, Alexandropolis, at the site of this defeat. This made his strength manifest to everyone and consequently became planetary of his fathers army. However, things still werent pleasing between father and son. On one occasion, Philip was attacked and hurt by rioters and he pelt and played shortly on the ground. Alexander shielded the attackers away and his father lived,but he neer acknowledged the fact that Alexander protected his life. Alexander deeply resented him for t his. Alexander was know for his many formidability tantrums. One unforgettable one was during a dinner companionship celebrating his final marriage. His uncle gave a toast reflexion that he hoped that they would corroborate a nipper to take over throne. Alexander replied, so am I just a bastard? and he threw his goblet at his uncle. This started such a great argufy between the two that Philip stood up and pull his sword at Alexander, but vicious over rummy before he got even close to him. To this Alexander yelled, Look, men,hes about to cross from europium to Asia, and he travel crossing from professorship to chair. Thisincident pressure Alexander and his mother to leave Macedon, but, some(prenominal) years later, they harmonise with him and moved back. However, concisely after their return, Philip was stabbed by the Captain of his bodyguards while he was entrance the theater that his nephews union was being held in. The assassinator fled across a vineyard. REA D papistical Aqueducts An Engineering magnificence Essay He might arouse escaped, but a vine caught his foot and he tripped. He was killed by some other bodyguards instantly. Philips death in 336 BC wind Alexander to succeed him and become King Alexander III. By this time, he was 20 years old. It was express that he was an incredibly handsome man and he invariably was clean shaven. Although he was a difficult drinker, he .

Saturday, June 22, 2019

Hockey and Culture Essay Example | Topics and Well Written Essays - 500 words

Hockey and Culture - Essay ExampleThe weight of capitalism as it diminishes the value of the sport is unmistakable in the discourse of hockey and how the same has become an industry manifested through capitalist agendas. For example, the strict traffic regulation in Gananoque which prohibits informal sports in the thoroughfare indicates to the stifling of grassroots sport. The push is on towards organization and commercial interests join the league, suffer a fee, buy this equipment, consume If you wont cooperate pay a fine (Genosko, p.239). Furthermore, an appreciation of hockey would be amiss without reference to its history which is marked by discrimination. Hockey was, in fact, the last North American sport to have Black athletes enter its ranks (ibid, p.235). The references to the white culture and the masculine stereotypes generally endure and prevail in hockey.But while there is non a shortage of informative and astute considerations in the essay, there remains the uniting factor that appeals to the emotional requisites that reasonably make sports endearing. Though athletes use their position while at the height of fame to maneuver into more lucrative endeavors after their hockey stints, Genosko does not fail to see how this is woeful rather than contemptible. Round, tepid, greasy provender sitting in pools of fat, like pucks on melting ice (Genosko, p. 233). The trend that inclines players to fast food has become evident. The same perception goes to the prohibition on the streets. This did not stop young people but instead fuels their imagination to create new landscapes and find other ways and venues to continue an immemorial tradition of uncase sports.The flow of the information was well developed and incorporates a number of aspects without going overboard by exhausting every detail.

Thursday, June 20, 2019

Frankenstein Essay Example | Topics and Well Written Essays - 500 words - 4

Frankenstein - Essay ExampleAs Victor is seen joining the university at Ingolstadt, he holds a monster, a grotesque act committed by him, which removes him far from the victory of committing a scientific triumph. He attempts to go beyond accepted human limits of acquaintance in order to create secrets not known to mankind. The story behind Frankenstein thus may be viewed as a lesson about the search for acquaintance as well as the dangerousness that accompanies the pursuit. The 1931 make the film based on this sci-fi thriller talks about how scientists at the time were not satisfied with what human brio had to offer. Victor was devastated and bereaved the death of his mother and soon began to create a human life in order to bring back those memories that he shared. However, his emotions got distort up within the scientific aspects of life and gave birth to destruction instead. He questions his friend, Robert Walton, Are you mad, my friend? Or whither does your senseless curiosit y lead you? Would you also create for yourself and the world a demoniacal enemy? Peace, peace Learn my miseries and do not seek to increase your own. Scientists have never given up on understanding the working of the human body, and just like most of them, Victor challenged himself to move his thoughts and ideas on to a more productive work sphere so as to not think about his past and family life. However, the film has a very tragedian note as it helps the audience understand the protagonists grief upon formulating a monster which ends up killing his loved ones.

Wednesday, June 19, 2019

WHAT IS ART Essay Example | Topics and Well Written Essays - 500 words

WHAT IS ART - Essay ExampleWe have creative art, conceptual art, abstract art, virtual art, representation, and other diverse make fors of art. Conceptual Art is where the intellection or concept is more important than the image. On the other hand, abstract artis a form of art that represents nothing. Most significantly, visual artsargon a form of art where an artist uses MEDIA and the available technology to communicate about the self to the entire world or audience. It is the most diverse form of art as it reflects the society. As such, art has a number of purposes that hold communication, entertainment, healing purposes, expression of imagination, propaganda among others. Art has various elements some of which are visual (Canley Davinci theme Web).There are numerous virtual elements of art that include color, form, border, texture, motion and time, shape, mass, volume, light, space, and value. Coloras a visual element of art has three properties that include the name of the c olor, intensity orthe purity and strength of the color, value, or the lightness or darkness of the color.Form on the other hand is a three-dimensional and encloses volume. In addition, time and motion are also visual elements of art. Motion subdivides to actual motion that is live movement, which displays actual motion when we see it in psyche and implied motion and time is a non-moving image that shows movement through the attributes present in the image. Consequently, the illusion of motion is what we experience when we see a movie or series of shapes that note a passage of time (Larmann Web).A lineis an element of art, which refers to the continuous mark made on some surface by a moving point. A line has measure, which is the length, and width of a line and expressive qualities of linethat define the individual character of a line. A line can be two dimensional, three dimensional, or implied.Shape, Volume and Mass

Tuesday, June 18, 2019

Communication Privacy Management Essay Example | Topics and Well Written Essays - 750 words

Communication Privacy Management - Essay ExampleThe research question for this paper was what are the ways in which the CPM theory can be used to enhance consumer management approaches in a business organization. This question seeks to explore ways in which organisations can build systems that can be used by its employees in identifying effective ways of collecting and managing consumer information. The question offers effective ways that can be used to ensure that the business manages to develop and manage consumer experiences for purposes of enhancing their loyalty in the business. The hypothesis for the research was organisations can build effective competitive strategies by ontogeny and marinating consumer privacy in their operations. This research hypothesis proceeds to examines ways that can identify the need to have such strategies for developing consumer privacy issues in the business. For this research, the respondents picked for the data collection process were invited i nto an on-campus lab for purposes of being interviewed on websites and sell music and other products using e-commerce. After getting consent to take part in the interview process, the respondents were disposed ten minutes to browse through the site and make some orders for music CDs and other products being sold. After the browsing process, the respondents were given questionnaires to fill and explain their experiences while interacting with the site.

Monday, June 17, 2019

The Thinker by Auguste Rodin Essay Example | Topics and Well Written Essays - 1500 words

The Thinker by Auguste Rodin - Essay ExampleOne of them is Franois-Auguste-Ren Rodin, popularly known as Auguste Rodin.He was a French sculptor, an artist with a sharp eye. His arts echoed until these days. He fashioned his deeds meticulously and looked at the details. Thus he was honored as the leading sculptor of the late nineteenth and early 20th century. He combined and artistically manipulated his works with detailed textures and modeling associating the vast emotions of man.The following year he decided to delve into doing ornamental stonework. And in 1862, her sister Marie died, which caused him great troubles and afflictions. He tried to enter the church but it was fate that brought him to met move Beuret in 1864 who became his life companion. Formally they were not conjugated by the matrimony of marriage until a few weeks before her death in February 1917 she was 53 years old then.The same year when he met Rose Beuret, he became an apprentice to the sculptor A.-E. Carrie r-Belleuse. His first submission to the official Salon exhibition in 1864, The Man with the Broken Nose, was rejected.In 1871, he went with Carrier-Belleuse to work on decorations for public monuments in Brussels. Carrier-Belleuse was not at rest with his work thus dismissing him. Soon, Rodin collaborated on the accomplishment of decorative bronzes, and Beuret joined him in Brussels.The rejection probably had made hi... Carrier-Belleuse was not satisfied with his work thus dismissing him. Soon, Rodin collaborated on the accomplishment of decorative bronzes, and Beuret joined him in Brussels.The rejection probably had made him to realize he needed to develop a direction of his own. It was consummated after four years he developed his own styles because he was urged to produce innovative and decorative works. In the midst of this innovation, He toured to flipper cities of Italy Genoa, Florence, Rome, Naples, and Venice before returning to Brussels. Italy has been the cradle of Rena issance art. He saw the works of Michelangelo and Donatello, amazed with their works he was inspired to create such fine works as Michelangelo and Donatello, bringing the emphasis on the muscle works and human emotions.He produced his first original work molded in bronze, The Vanquished, depicting dire expressions of a physically overpowered man seeking for rejuvenation. Scandals arose in his exhibition in Brussels and at the Paris Salon of The Age of Bronze in 1877. race did not believe that he made such realistic work and he was charged of casting it from a living person.His former master perceive that he went back to Paris in 1879, Carrer-Belleuse asked him for designs.After much controversies and rejections, he was granted a payment and appointed to make a statue for the City sign of the zodiac in Paris. He gained his reputation as a sculptor in 1880 with the success of his sculptures The Age of Bronze and St. John the Baptist Preaching. The same year he imitate The Gates o f Hell, the sculptured bronze door for the Muse des Arts Dcoratifs in Paris. The door incorporates scenes from The Inferno. It was made through the inspiration of Dante Alighieris The

Sunday, June 16, 2019

Effects of the Westward Expansion of the Colonies of the United States Essay

Effects of the Westward Expansion of the Colonies of the United States - Essay ExampleGradually the Americans moved far into the western United States in search of fertile lands irrespective of being converts to Christianity or having religious freedom. They even began trading with the Indians, which led to several treaties with them. Inspite of these peace treaties war continued as the Indians hardly assumed to be bounded to treaties and the US government was loyal to its duty of protecting the movement towards the west. In 1848 San Francisco was a small region, which grew into a king-sized city based on rapid commercialization, and industrialization while the mining industry became popularized. The move towards the west was initiated owing to a search for new and amend opportunities in terms of land for farming and jobs to survive. The Midwest became the attractive zone for the settlers and the industrial revolution encouraged commercialization. The proprietors of the lands bega n to promote their commodities by proving adequate connectivity with the east. As the timbre areas became reduced in the east during 1849 the lumber industry moved westwards to Michigan and Minnesota. This would ensure they were not moving away from civilization. Again, the professions gradually shifted from farming to some other activities like lumbering and mining. Industrial revolution brought about machineries, which saved labor, and farming became smoother.

Saturday, June 15, 2019

Enterprise and business development Essay Example | Topics and Well Written Essays - 500 words

Enterprise and business development - Essay ExampleFirestarter considers the supplier dynamics and aims at positioning itself in a position of relative advantage in a bid to maintain or increase its node base. Supplier will become powerful if they are few and there is a heavy need for them to facilitate the main processes (Porter, 2008).The buyer power is a consideration that the competitive players in the food industry consider. The buyers have the ability to drive the prices down thus influencing the ability of firms such as Firestarter to dictate set terms. The number of buyers in the specific market ventured by Firestarter and separate listed competitors, the replacement cost of a buyer and importance of that buyer to the enterprise influence the vex of strategic planning on pricing so as to keep at bay with the competition. Firestarter may be able to dictate prices if the cost of switching to the other suppliers is high and the presence of few powerful buyers.The capabili ties of the competitors in this context, KFC, Costa, Dunkin Donuts, Starbucks and Muzz Buzz form a competition rivalry. It is through differentiation of products and services more than the other that one individual farm can achieve an advantage. For the case of Firestarter, offering a variety of products that are differentiated at better prices will shed light on it have an edge over the competitors in the niche by achieving market attractiveness to the consumers in the U.K.Entering a new market poses threat specially in the event of high costs and time in entering the market (Porter, 2008). To be cherished against compromising of a favorable competitive position, enterprises such as Firestarter ought to have economies of scale in the market and have protection of their innovations in products and services. This is a key consideration to be employed by Firestarter to protect itself from new entrants such as Muzz Buzz. Maintaining of durable and solid barriers to entry such as a st rong and loyal base of consumers,

Friday, June 14, 2019

Health informatics Research Paper Example | Topics and Well Written Essays - 1500 words

Health informatics - Research Paper ExampleIt requires valid techniques and caution in parliamentary procedure for success to b realized. The definition of health is refers to the state of an individual having no illness or any injury affecting them. The definition of informatics is refers to the perception found in the computer information systems. These two words come together to bring benefit in the healthcare sector. However, health informatics has its profess importance, advantages and disadvantages.Health informatics is an classical part of the health sector in conglomerate ways. For one, through health informatics, important enquiry can be undertaken on serious diseases that trouble many patients. For instance, research on diabetes which affects both adults and children is taken to another level as they try to find ways of making this fit treatable using less harmful treatments. For his reason, having health informatics personnel is important in any clinical institution as it aids in the research department. These personnel will be able to guide the researches using their information technology expertise on how to go deeper in their research on various subjects (Hersh 2009). They will be able to enable the institution identify what might have been missed in the past. This is because they will be using much ripe technology in their research.Health informatics personnel are also important in the clinical sector due to the involvement of several important areas. Some of these areas include reminder systems, electronic health records, data repositories, coding, ontologies and vocabularies as well as data sharing. When such advanced factors are put in the clinical sector of any healthcare environment, then the quality of their health provision is advanced. Communication is made much easier. When the individual in charge necessitate to confirm a certain concept then they can easily access the materials due t easier

Thursday, June 13, 2019

Lab #7 and #9 Assignment Example | Topics and Well Written Essays - 500 words

Lab 7 and 9 - Assignment Example still structural and functional abnormalities have been observed in this area in schizophrenia patient. Therefore it was suggested that schizophrenia patients may have selective attention problems. This report was conducted to test the hypothesis patients with schizophrenia would fail to activate the anterior cingulate gyrus during selective attention performance.Fourteen schizoid patients and fifteen age and sex matched powerful subjects were recruited for the experiment. They were instructed to perform a computer based stroop task in which they have to tell the color of the letters of different lecture while their brain act was scanned by positron emission tomography. During the experiment answers of the participants and time taken to answers were recorded to the nearest millisecond. Three types of words randomly displayed on the computer screen. 1. Color matched words (eg. red in red color), 2. Color un-matched words (eg. red in blue color) and neutral words (eg. car in blue color).There was no significant difference between the schizophrenic and healthy groups in time taken to answer. However error rate was significantly higher in schizophrenic patients than that of the healthy group (11.1% Vs 1.9%, pObesity increases the risk of mortality and morbidity chronic diseases such as diabetes and stroke. Although excess energy intake directly contributed to fleshiness some animal experiments have shown a relationship with diet composition. This cross sectional study was

Wednesday, June 12, 2019

American Conflicts Research Paper Example | Topics and Well Written Essays - 7500 words

the Statesn Conflicts - Research Paper ExampleThe revolution was seen as a war of independence. There are some(prenominal) stages in a revolution. One stage is that the various fibers are knit together and you have to stretch some parts while loosening the separate parts. There can several analogies of a revolution. But one thing is certain, there has to be changed. Revolution is changing, and the change can be in the system, in the method and the ways of ruling. The social consequences of a revolution are not the result of the wishes and plans of those who started the revolution but by the desires of those who are in control. The the Statesn Revolution was started by only a few compared to the population at that time. It was started by a numerical minority, just like the Viet Cong, although by the end of the revolution, the majority of the colonists whitethorn have supported the struggle against Great Britain. The war between the Patriots and Tories resembled like the Vietnam-Vie tcong conflict. The Americans sought foreign aid instead of foreign alliance. John Adams warned that alliances would entrap America in future European wars. For instance, the French did not want a second American invasion of Canada, and the French did not want the new join States full control of the North American continent because they had the fishing rights in the Mississippi.Wars mar the history of a nation (Morris, 1969, p. 350). The Civil War in Americas history, which marked its 150th year in 2011, tested the American spirit.

Tuesday, June 11, 2019

Are humans born with a conscience will send assignment sheet for 300 Research Proposal

Are humans born with a conscience will send assignment sheet for 300 aim english class - persuave writing - Research Proposal ExampleWhat he is saying is that many theologians believe that some people are born with the skill to be good, and others arent. Paul doesnt agree with this assessment. He believes that we all have the capacity to be good, because God gave it to us. While he also believes that one must love to do good and avoid evil, (120) the fact is that whether we choose the right road has nothing to do with the fact that Gods grace has tending(p) us all the possibility to always choose good. Paul added, Conscience is not an infallible judge it can make mistakes (121). This is obviously true. The Bible teaches us that none are perfect, and that all fall short of Gods glory. Still, Paul maintains that the important thing is to know the difference in what we choose, and what we have the power to choose. While it is clear to Paul that we have, innately, all we pack to mak e good decisions, we still need to study the Bible, and attend, church as a compass to give us headed in the right direction.In Lois Walkers Religion Gives Meaning to Life, Walker writes about a similar belief. It is her opinion that who we are is obstinate at the time of our births. She writes, We are not the products of chance (626). Walkers essay is in response to an atheist who claims that people only need to physical exertion being responsible to know the right thing to do. According to Walker, the atheist proclaimed, We dont need a big Daddy in the sky. We need to grow up and become our own parents (624). While we do need to know how to govern ourselves, we do, also, need our Father in heaven. Walker is states that she believes in the theist philosophy that holds, The universe is suffused in goodness and that good will win out over evil (627). Gods purpose, according to Walker, is to give us a continuous reason to want to behave. If God can give his son

Monday, June 10, 2019

Response Paper Essay Example | Topics and Well Written Essays - 500 words - 3

Response Paper - Essay ExampleThe arguments presented by the defense about the murderer atomic number 18 mere excuses. If the argument is to entertain true for this valet, what would be the explanation for the same situation if an all-round nurtured individual is involved? There are individuals who are brought up by loving parents, provided with anything they desire, rise up educated, and in good careers who still engage in similar or worse crimes. In the event that the argument is considered viable, then individuals who are brought up in favorable environments should never commit crimes. However, this is not the case. People who engage in crime exhibit all kinds of backgrounds good and bad. In this regard, this man is much little of an environmental product.Wallowing and self-pity best describes the personality and character of this man. The man has totally accepted that the state he is in is the best life can offer. He, therefore, views every other(a) thing that happened to h is life in the past as a complementing factor to the situation. The society cannot be accused of neglect when the defendant has not been said to ca-ca tried to get some help from charitable agencies. The situation is more of a choice than a result of the unfolding events. As a result, this man is fully responsible for his actions. While the fact that the man has had a difficult past cannot be disputed, there are other alternative choices available to him than taking his past as an excuse in the situation.It is openly evident that all persons that have been previously charged with the crimes outlined in the situation did not inescapably share the same experiences with this man. For instance, drugs are expensive, and even the rich and wealthy engage in drug use and end up addicted to these drugs. There are others who have realized their wealth from robbing and related actions. In other words, the experiences of this man

Sunday, June 9, 2019

The African-American Artists Essay Example | Topics and Well Written Essays - 500 words

The African-American Artists - Essay ExampleI firmly conceptualise that the success of a song or musical composition does not entirely depend on the ethnic, racial or cultural background and penchant of the artificer. Other factors contribute to the success of a musical composition, including the integrated components of musical elements such as the harmony, pitch, lyrics, melody, and rhythm, among others. Music, interpreted by a particular artist, regardless of zip or other personal differences in gender or age, would have found equal success with the right time, right musical score, choice of instruments, vocals and the communicative interpretation of the artist that elicited the appropriate appeal of the target audience.There are different instances when songs have been initially recorded by one artist and have been interpreted by another. For instance, the song of Josh Groban entitled You Raise Me Up was popularly known to have been recorded by various artists blush before it rose to fame when Josh Groban recorded and sang it. The reasons why thither are versions that generated significant popularity and success more than others who previously recorded them could range from the timing of these songs being recorded which perfectly match the sentiments and appeals of the audience the personality of the singer or artist who sang and interpreted the song (where it could be observed that there are a particular appeal and charisma that was exemplified more than what the other artists who previously recorded them delivered) the strategies used for promoting the songs and there could be whatsoever changes in some musical elements, like the tempo, rhythm, and musical instruments or accompanying background that made a particular musical interpretation more successful than others.

Saturday, June 8, 2019

I am not sure about the subject that i used, but you have to put the Essay - 1

I am not sure about the subject that i used, only when you have to put the right subject for my work - Essay ExampleGeological studies, which deal with the details of the formation and structure of the physical geography, stand as a basis for analyzing the characteristics of places selected for the scientific observation to find out their fitness and potential threats. The Arbuckle Mountains in the North American constitute oneness of the prominent geographical locations for the geologists to make experimental studies based on its history and the formation structure. This paper will make a comprehensive trial for investigating the geological history of the Arbuckle Mountains on the basis of the geographical specifications of this region in the Oklahoma state of the United States of America.Like the presence of a diverse culture among its population, the United States in like manner has the characteristics of accommodating varieties of geographical patterns inside its political b oundaries. They include a number of large and small rivers, forests, plains, plateaus, mountains and valleys besides the urbanized industrial regions and the vast areas of land infra the cultivation of numerous edible and commercial crops. Generally, mountains get the quickest glance of every explorer of a region because of the attraction they yield with their elevated altitudes. For the geologists, they obligate essential information about the nature of the geographical structure and thus help them make necessary assessment of the potential challenges and advantages before the plains and valleys associated with the mountains. The Arbuckle Mountains are one the oldest mountains discovered in the United States, and therefore, it is important to know the details about the regions geological specifications by forming an overall idea about these mountains.The Arbuckle Mountains repose in the south-central region of the Oklahoma state in the United States of America (Have you thought about Oklahomas Climate And geography?). As Oklahomas geography features a large number of mountain ranges, the state

Friday, June 7, 2019

The Crash of American Airlines Essay Example for Free

The Crash of American Airlines EssayA good example involving with human error is the crash of the American Airlines fledge 587. On 12 November 2001, at approximately 917 a. m. ocal time, American Airlines flight 587 crashed into the Belle Harbor area of Queens, New York, several minutes after victorious off from JFK International Airport. The plane was on a scheduled flight to Santo Domingo, Dominican Republic. All nine crew members and 251 passengers on the aircraft were killed, including five infants. Five plenty on the ground were also killed. Let examine the summary of events that the NTSB determined led to the crash in order to identify the probable causes. The A300-600, took off scarce minutes after the japan Airlines Boeing 747 on the same runway, flew into the galacticr jets wake, an area of very turbulent air.The first officer attempted to keep the plane adept with aggressive rudder inputs. The strength of the air flowing against the moving rudder stressed the air crafts vertical stabilizer and eventually snapped it off entirely, causing the aircraft to lose control and crash. The National superman Safety Board concluded that the enormous stress on the rudder was due to the first officers unnecessary and excessive rudder inputs, and not the wake caused by the earlier Japan Airlines 747 that had crossed that area.Airbus charges that the crash was mostly Americans fault, because the airline did not train its pilots properly about the characteristics of the rudder. Aircraft tail fins are designed to withstand full rudder deflection in one direction at maneuvering speed. They are not usually designed to withstand an abrupt shift in rudder from one direction to the other. Most American pilots believed that the tail fin could withstand any rudder movement at maneuvering speed. The NTSB indicated that American Airlines Advanced Aircraft Maneuvering Program tended to exaggerate the effects of wake turbulence on large aircraft.Therefore, pilots were being trained to react more aggressively than was necessary. (Mysterious Crash) Since the investigation by the National Transportation Safety Board, American Airlines has modified its pilot training program. homework methods have been reviewed and changed as a result of the accident. Despite rapid gains in technology, humans are ultimately responsible for ensuring the success and safety of the aviation industry. They moldiness continue to be knowledgeable, flexible, dedicated, and efficient while exercising good judgment.

Thursday, June 6, 2019

Learning and Development Practice Essay Example for Free

Learning and Development Practice Essay1. Directive Is where the coach offers the coachee solutions, tools and techniques for abject forward. The coachee whitethorn like to be offered solutions however the danger is that the solution may not be appropriate for the coachees situation and consequently may not feel fully committed to the solution provided. 2. Non-Directive Is coaching job job in the true sense of the word where the coach simply asks the coachee questions to allow the coachee to find your own solutions. A non-directive coach will not offer the coachee advice and rarely even give the coachee suggestions, although through skilful questioning they will help the coachee to see their situation from a different perspective, gain clarity, uncover options, challenge inconsistencies and hold the coachee accountable to their actions. Directiveness ScaleDirective Non-Directive I know how You know how I tell you You tell me You observe instruction- You decideStarr, J. 2008 . The Coaching Manual. Person Education LTD. Pg20. Although I have given an idea of what directive and non-directive coaching styles are. These styles can be seen on a slide scale. A tool for the coach to use, dependent on the situation and where the questioning leads. The coach my not always use one style over other but can slide up and down the scale to enhance the coachees answer and gain clarity where needed, or dependent on the have intercourse of the coachee. With the ultimate aim that the coachee leaves empowered to move forward with action points to achieve goals. 1.2 How coaching differs from other LD methods adviseCounselling a therapeutic intervention usually around a personal deep rooted issue from a persons past that is affecting a person in the present. Counselling provides intervention strategys to cope with the personal issue by delving into the persons past. Coaching although can bring out emotions from the coachee, tends to be forward looking and is establish a round action related issues and not personal. (Beevers, 2010).1.3 How coaching can meet organisational objectivesCoaching can meet organisational objectives byStaff engage and have a greater awareness of organisations objectives. Implemented agreed actions during coaching process show recorded outcomes which means the organisation has measurable results of learning within ply PDPs. Shows an organisation has a learning culture investing in their people. If coach is line manager they will enhance management capabilities within the organisation.1.4 Coaching rolesThe Coaches roleEstablishes the boundaries, e.g. frequence and length of sessions as well as the session structure. Explains what coaching is and is not, and asks permission to explain when issues go beyond what is permissible in coaching. Helps the coachee set goals through questions. screening interest, activity listening, being non-judgmental. Shows confidence in coachee to find own solutions. Helps coachee gain insight through questioning, listening and challenging them. Encourages forward movement and thinking.Helps coachee set judicious goals and feedback on those goals.Holds coachee accountable for his/her own progress and does not own the actions.The Coachee roleCommits to the coaching process and be an active part.Takes ownership of their progress.Is honest, open and shares information with the coach. ordain to discover more self awareness.Takes ownership and willingness to move forward with agreed actions to achieve goals. Understands that the coach is only human and that mistakes from both the coachee and the coach may be made along the journey.1.5 The benefits of coachingIndividualIncreased Confidence and self awareness from developing own solutions and goals (ILM,2007). Dedicated prison term to discuss own performance and ownership on how to improve it. Better understanding of their contribution within their role related to the organisations objectives.OrganisationMotivated staff leadi ng to better staff retention throughout the organisation. Improved communication and relationships between management and staff. Improve business knowledge and skills in specific areas related to the organisation (ILM, 2007).1.6 How to implement a coaching culture within an organisation Bringing in external coaches would be dependent on cost External coaches are costly, although may be more dependent and more reliant to complete agreed actions, but are more likely to be used short term and teeny-weeny scale (Beevers, 2010). External coaches are more likely to be brought to coach executive level management (Beevers, 2010), or to train up in-house coaches who can wherefore coach at lower levels of management and/or line managed staff within the organisation as part of the development process (Harrison, 2009). 1.7 Developing in-house coachingAdvantagesCoach will have existing knowledge of the organisation and understands the organisations objectives. Can be more cost effective when c oaching a large hands in comparison to bring in an external coach. As a manager/coach can offer immediate coaching to team members when issues arise.DisadvantagesCoachee may not be willing to open up to coach about issues, especially is coach is their line manager due to lack of trust of confidentiality. contravention of interest if coach is a manager They have their own targets to achieve which might affect the aims or outcome of the coaching session. Costs of training, supervising and time from normal workload to coach if internal employees are trained to become coaches (Harrison 2009).ReferencesBeevers, K and Andrew, R., 2010. Learning and Development Practice. CPID. Harrison, R. 2009., Learning and development. 5th edition. CIPD. ILM. May 2011., Creating a coaching culture. ILM.Starr, J. 2008., The Coaching Manual. Person Education Ltd.Advantages and disadvantages of different types of coaching relationship Online Available at Accessed 31/09/13.

Wednesday, June 5, 2019

Impact of Instagram on Social Networks

Impact of Instagram on Social NetworksHow Instagram makes us sociableIntroductionThe world has really transformed in foothold of applied science. Technology is the only thing that changes at a very fast rate. Every day we wake up to a new innovation of applied science and ideas. Technology through and through internet has managed to bring the world to draw a bead onher in terms of communication. notwithstanding the varying time zones in different parts of the world, mesh has paved way for Social net profits that are turning be a vital field for advertising and creating awareness among heap. The internet is a branch of technology where there is loads of traffic. Internet has now be capture a necessity and has managed to globalize the universe and make it as a small village where people interact freely despite the location and time of the specific region. It has as well given rise to several cordial networks that allow people to adhere joined and in impinging at any time of their convenience. Some of the well- cognize neighborly networks include Twitter, Instagram and Facebook. These kind platforms have allowed business people to give notice (of) their companies as well as the products being marketed.This research paper pass on focus on how Instagram has managed to make people more sociable. The previous notion was that kind networks are responsible of making people lonely and create an ambience of loneliness. It depart shade more light on how Instagram brings peoples affable life into one stage where each and every individual is required to register an account with so as to be given the unique security for logging in and interact with other subscribers. Businesses are now turning to favorable Medias to advertise and promote their sales. This is because of the ever increasing favorable traffic which is ever so present regardless of the time or the place of business. Instagram has remarkable improved sociable exposure and this research paper will conclude on its splendour shankd on purely situations and figures.ThesisThe Research topic that this paper is going to investigate in detail is whether Instagram as a Social network has managed to make people more sociable rather than lonely. My stand on the above argument is that I personally believe and completely agree to the fact that Instagram has truly managed to make people more social as compared to before the beginning of social networks. In order to second my stand in opinion, we will use a demonstrative example trying to lawsuit for an election contestant who is vying for an elective sit. This will put us in a billet able to compare the different forms of campaign and establish the one which managed to reach the most number of people. For my research, I will employ Instagram as one of the trending social networks to establish whether it is making people to be more social rather than lonely.Before putting keen emphasize to the example above we send packing exac tly ask ourselves as to why businesses are also targeting the social population as a potential field for advertisement. This is just a way of proving that Instagram (one of the social networks) has made people more sociable and has improved communications. Loneliness on the other hand can be defined as being marooned from the other members of the population and still not in a position to go by in touch with them. A lonely person is one who cannot keep in touch and communicate with others at free will. How can Instagram promote loneliness from our definition of the word itself? Instagram has managed to allow people keep in touch hence my support for the Research topic.Literature ReviewSocial network has been essential in changing the internet from a space widely known for broadcast of information or transmission to a place where users are involved in creating and generating their own personal content. This ranges from short films on You Tube to Facebook updates. This is and so fol lowed by the process of signposting their networks to the other contents created by other users (Beer, 2008 Thelwell, 2009). For literature purposes, there is a very clear attention on the extent to which social media has become transformative through the way that users keep in touch with one another. Evidences from several sources around that could be found with less difficulty show that campaign and influencing is the most effectively done activity through peoples already existing social relationships (Alinsky, 1971 Freire, 1970). Despite the fact that all social media platforms prevail a major role in this, there is a general sense in which social networks have increasingly become powerful. The masses that can be reached within a given short issue of time via social networks are overwhelming. One post with no duplicate of exactly the very(prenominal) information can be viewed and followed by thousands if not millions at a blink of a second.Most of the already existing social networks were initially open up on the basis of a more tailored proposition rather than merely creating an online space where people with common interest could interact freely. My space, for instance, is a social network which was initially started with the sole purpose of enabling aspiring music artistes to share and publicize their own piece of work with other likeminded people among the society. (Pace, 2006). Facebook started as a mere service for students in a U.S university which gradually broadened their service to other non U.S universities and this is even before making the platform accessible to everybody (Kirkpatrick, 2010). This is how social networks manifestly start and end up being a populated platform with millions of users all over the world.Social network sites provide new audience for polite engagement and for political participation. This facilitates information sharing and brings together networks through actions such as utilizing email, networking practices a nd user generated content (Montgomery 2007Vromen 20072008). Research studies in Saudi Arabia have shown that 38% of 17-19 year olds employ Social networks (Instagram) for both political and civic engagement (Smith et al. 2009). Political leaders are using Instagram to post images of peace that advocate for peaceable coexistence. The many followers the politician has in Instagram media platform will be able to see what their leader is promoting and even leave a comment if they advert to. Moreover, the followers can get the images from the Politicians profile and put it as their own so as to spread the message. This is just a saucer-eyed example of how Instagram has enabled leaders in Saudi Arabia to campaign within a short stream of time.For the young generation who do not think through their participation in either civic or issue-based functions as political in the institution or traditional sense, Social networks are used to connect the individuals who have similar interests a bout their own projects (Collin, 2010). Young people are coming up with new participatory communities which are by and for their peers. (Harris 2008489 Boyd 200713-14). In addition, service and organizations are increasingly observing social networking practices as the succeeding step towards youth participation in community and government decision making (Collin 2010).Despite the fact that there are still queries as to the extent to which social networks function as virtual public spheres, fostering deliberation and enabling place participation (Vromen.2008). However, the network for communicating information and numerous point of view Public sphere permits the recognition of creative digital expression through the social networks as civic engagement (Burgess, et. al,2006).There is a vast body of academic work on social networks and technology at large which make into circumstance the creative, performativity and participatory dimensions of the current digital practices.The gene ral usage of social media can simply be described as the collective media creation and sharing on a legitimately large scale. This includes social network services as well as other participatory activities like news blogs. The rapid up take of both social media and social network services by the young people indicate a very important shift in young generations use if internet mainly for information and entertainment as one way of communication. The young generation is made of producers who actively produce as well as consume media (Bruns 2008). The participatory media ambience enables the young to take part in creative content production. This also empowers them with dishonor new means of creating as well as sustaining connections among themselves. As a result, Social network platforms have resulted into new discussions on how to conceptualize and advocate for what has come to be termed as cyber citizenship (Bennett, 1998, 2003 Coleman, 2005).Research MethodologyAs mentioned earlie r on in this research paper, I will use a politician social network campaign program via Instagram to establish the power of the social platform in terms of moving masses within a short period of time. I will visit colleges and higher institutions of learning and promote students to register accounts with Instagram. This on the other hand will simplify my work because I watch only few students if any who will not be having accounts with Instagram. I will then advise all the students to follow the Politians account so that they can be able to be informed about the Politian updates in his process of campaigning.I will collect my data for depth psychology by computation the number of likes and dislikes for each and every update the politician makes. The number of likes will give me a rough idea before the actual data analysis whether the social platform is in support with the campaign trend or not. Reading through the comments left behind by the followers will financial aid me to co nclude the factual side of support or critic from the public. The advantage of this methodology is that it comes at no extra cost to the students since opening an Instagram account is predominantly free and there is free access to internet services in campuses. I will collect all the data throughout the campaign period and code it statistically and appropriately while observing all the statistical hypotheses.In collaboration with the election contestant, I will pose some questions that call for discussions among the respondents (students on Instagram). This will create a thread of comments and opinions from the online traffic generated. I will then read through each individual comment and take it as the opinion from the respective person. Depending on the number of opinions and comments that I will get, I will decide on picking a representative try on of the stallion online Instagram traffic. This sample will be free from biasness and will be selected using a statistical formula called Fishers et. al. I will base on factors such as age, gender and level of education to ensure that I come up with a good representative sample of the entire school Instagram accounts. . (Raatma, L. 2010)I will employ systematic random sampling technique to allocate my sample. This will be because of some factors that I find indispensable to be observed. These factors will ensure that the selected sample is a true representation of the population at large and inferences drawn from it will be descriptive and enlightening about the population. I will ensure accuracy of the data by designing self-explanatory research questions that can be easily answered by the online Instagram users. (Raatma, L. 2010)I will analyze the data using one of the statistical softwares namely S.P.S.S. (Statistical Package for Social Scientists). I will compare the levels of campaign awareness from different Medias of communication with the sample data I will get. These other Medias of communications ca n be via bill boards advertising, television news and radio. To get information on previous voting exercises, I will go through secondary data and reports that are available in file. I will analyze the data through diagrams such as pie charts, bar graphs and line graphs.ReferencesAndrews, L. B. (2012). I know who you are and I saw what you did social networks and the death of privacy. natural York Free Press.Barnes, J. A. (1972). Social networks. Reading, Mass. Addison-Wesley Pub. Co..Barnett, G. A. (2011). Encyclopedia of social networks. Thousand Oaks, Calif. Sage.Buskens, V. W. (2002). Social networks and trust. Boston Kluwer Academic Publishers.Gottlieb, B. H. (1981). Social networks and social support. Beverly Hills, Calif. Sage Publications.Kilduff, M., Tsai, W. (2003). Social networks and organizations. London SAGE.Lin, N. (2001). Social bully a theory of social structure and action. Cambridge Cambridge University Press.Maguire, L. (1983). Understanding social networks. Be verly Hills Sage Publications.Raatma, L. (2010). Social networks. Ann Arbor, Mich. Cherry Lake Pub..

Tuesday, June 4, 2019

Determination of Protein Concentration by Uv Absorption

Determination of Protein Concentration by Uv AbsorptionHighly susceptible to contamination by buffers, biologic materials and saltsProtein amino dosage composition is extremely important, thus the choice of a standardized is very difficult, especi altogethery for purified proteinsAbsorbance is heavily influence by pH and ionic strength of the solution. frequent ConsiderationsThis is often accustomd to estimate protein concentration prior to a more sensible method so the protein give the bounce be dilute to the correct effigyQuantitative Procedure adjust the spectrophotometer with a buffer blankMake a standard curve using your standard of choice in the expect concentration range, using the equivalent buffer that your unknown audition is in.Take the absorbance values at 280 nm in a crystallization cuvettePlace sample into quartz cuvette (make sure concentration is in the range of 20 g to 3 mgTake absorbance at 280 nmEstimation ProcedureZero spectrophotometer to piddle (or b uffer)Take the absorbance at 280 nm in a quartz cuvetteChange wavelength to 260 nm and zero with water (or buffer)Take absorption at 260 nm in a quartz cuvetteUse the following equation to estimate the protein concentrationProtein (mg/mL) = 1.55*A280 0.76*A260DiscussionDetermination of protein concentration by ultraviolet absorption (260 to 280 nm) depends on the presence of aromatic amino acerbs in proteins. Tyrosine and tryptophan absorb at approximately 280 nm. Higher orders of protein structure also may absorb UV fall down or modify the molar absorptivities of tyrosine and tryptophan and thus the UV detection is highly sensitive to pH and ionic strength at which measurement is taken. some(prenominal) other cellular comp championnts, and particularly nucleic blisterys, also absorb UV light. The ratio of A280/A260 is often used as a criterion of the purity of protein or nucleic acid samples during their purification. The real advantages of this method of determining protein c oncentration are that the sample is not destroyed and that it is very rapid. Although different proteins bequeath have different amino acid compositions and thus different molar absorptivities, this method can be very accurate when comparing different solutions of the same protein.To make an accurate determination of protein concentration, you will have to produce a standard curve (A280) with known amounts of purified protein. You will also have to provide a blank that is appropriate for the sample and contains the same concentrations of buffer and salts as the sample. It is often convenient to dialyze the sample and measure the absorbance of the retentate (still in the dialysis sack) using the dialysate as the blank. Care must be taken to use quartz cuvettes, since glass absorbs UV light. A handy equation to estimate protein concentration that is often used isProtein (mg/mL) = 1.55*A280 0.76*A260However, it is also a good idea to endlessly use a standard curve and suggested that you evaluate the agreement of the results using the above equation with results using a standard curve.This method is the least sensitive of the methods discussed here. For increased sensitivity, the wavelength can be lowered to the range of 210 to 225 nm. This measures the amide bond in proteins. However it is much more subject to interference from many more biological components and compounds used to make buffer solutions.If you dont know what the protein concentration of an unknown sample is likely to be, the ultraviolet method might be a good starting line point. Prepare a standard curve for the absorbance at 280 and 260 nm. After you have the data for the standard curve, rezero the spectrophotometer with water. Place your samples into a dry 1 mL quartz cuvette and read the absorbance. If the A280 of your unknown sample is less than 2, you should probably not dilute your sample further. If the absorbance is 2, dilution will be required. When you are finished with the first mea surement, the unknown can be returned to its airplane pilot tube with minimal loss.The Lowry AssayReferencesO.H. Lowry, N.J. Rosebrough, A.L. Farr and R.J. Randall (1951) J. Biol. Chem. 193 265. (The original method)Hartree E. E. (1972). Anal. Biochem. 48 422 (This modification makes the assay additive over a larger range than the original assay)J.R. Dulley and P.A. Grieve (1975) Anal. Biochem. 64 136. (This is a useful modification of the original Lowry method that includes 0.5% atomic number 11 dodecylsulfate in the alkaline reagent. This obviates interference from many detergents and helps disperse membranes in the sample.)A. Bensadoun and D. Weinstein (1976) Anal. Biochem. 70 241. (Another useful modification of the original Lowry method that can be useful when the solution contains interfering contaminants. The proteins in the samples are precipitated by a mixture of sodium deoxycholate and trichloroacetic acid and centrifugation prior to assay. If the contaminants stay in t he supernatant they can be removed and the amount of precipitated protein determined.Quick GuideHow does it make for?The first step is a Biuret reaction which reduces Cu+2 to Cu+1The second reaction uses Cu+1 to reduce the Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate). This is detectable in the range of 500 to 750 nmDetection Limitations2-100 gAdvantagesSensitive over a wide rangeThe most commonly referenced procedure for protein determinationCan be performed at room temperature10-20 times more sensitive than UV detectionCan be performed in a microplate formatDisadvantagesMany substances interfere with the assay base-forming pig reagent is laborious to prepare and will develop carbonate scales over storage which interfere with optical activity, thus it must be prepared gratifying dailyTakes a considerable amount of time to performThe assay is photosensitive, so illumination during the assay must be kept consistent for all samplesAmount of color varies with diffe rent proteinsGeneral ConsiderationsSome researchers have reported that repeated assays in the same cuvettes cause them to be etchedMany chemic distributors sell a modified Lowry assay that is more stable and sensitive than homemade versionsSince reduced copper is detected in the procedure, make sure that the distilled water used in the procedure is fed from plastic lines and not copper lines. In general water from 18 megaohm water polishers is satisfactoryVariation in the content of tyrosine and tryptophan residues will influence the assayProcedureAlkaline Reagent0.1 M NaOH2% Na2CO30.5% Na Tartrate (use of potassium salt will cause SDS to be insoluble)0.5% Na DodecylsulfateCopper Reagent1% CuSO4*5H2OAssay Mix (MAKE FRESH EACH DAY)50 mL alkaline reagent and 0.5 mL copper reagentFolin-Ciocalteu ReagentDilute with an equal hatful of water to prepare the desired ledgerProcedureAdd samples containing up to 100 g of proteinBring all tubes to 1 mL total volume with water. Be sure to hav e two tubes containing only water as blanks. Also use reagent or buffer blanks if needed.Prepare the Assay Mix and diluted Folin-Ciocalteu reagent.To each tube add 5 mL of assay mix and thoroughly vortex.Incubate tubes at room temperature for 10 min.Add 0.5 mL of diluted Folin-Ciocalteu reagent. Vortex immediately.Incubate at room temperature for 30 min.Vortex the tubes, zero the spectrophotometer with the blank and measure absorbance at 660 nm (or other appropriate wavelength). The data from the standard curve are usually linear enough that a straight-line interpolation can be used to determine the concentration of unknowns.DiscussionThe Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds, forming reduced copper in alkaline solutions. This is called a Biuret chromophore. The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate) by tyrosine and tryptophan residues. The reduced Folin- Ciocalteu reagent is blue and thus detectable with a spectrophotometer in the range of 500-750 nm. The Biuret reaction itself is not all that sensitive. Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone.The assay is relatively sensitive, but takes more time than other assays and is susceptible to many interfering compounds. The following substances are known to interfere with the Lowry assay detergents, carbohydrates, glycerol, Tricine, EDTA, Tris, potassium compounds, sulfhydryl compounds, disulfide compounds, magnesium and calcium. Most of these interfering substances are commonly used in buffers for preparing proteins. This is one of the major limitations of the assay. The Lowry assay is sensitive to variations in the content of tyrosine and tryptophan residues. If the protein you are assaying has an unusual content of these residues, an appropriate substitute standard is required. The standar d curve is linear in the 1 to 100 ug protein region. The absorbance can be read in the region of 500 to 750 nm. Most researchers use 660 nm, but other wavelengths also work and may reduce the effects of contamination (e.g. chlorophyll in plant samples interferes at 660 nm, but not at 750 nm).